Histology, tissue morphology and microscopy

Study design

• Assistance in project planning and experimental set-up based on scientific question
• Evaluation of samples, photodocumentation, and report writing

Histological preparation and techniques

• Undecalcified bone histology with or without implants (eg, metal, calcium phosphates, polymers)
• Sectioning of resin embedded samples with specialty equipment
• Contact radiography
• Decalcification of tissues
• Paraffin embedding and sectioning
• Cryosectioning
• Immunohistochemistry (with protocol planning)
• Routine to special stains (for resin and non-resin embedded samples)

Microscopy and image analysis

• Brightfield light microscopy for sections with or without implants
• Epifluorescent microscopy for fluorochrome labelled sections (eg, bone labels, marked antibodies)
• Macrophotography for overview images of larger samples
• Confocal laser microscopy for optical sectioning
• Scanning electron microscope equipped with Energy Dispersive X-Ray Spectroscopy (EDX or EDS)
• White light profilometry for surface characterization (eg, roughness values)
• Quantitative analysis (eg, lengths, areas, numbers) on histological samples